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KMID : 0525720110160030197
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Journal of Chitin and Chitosan
2011 Volume.16 No. 3 p.197 ~ p.203
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Characteristics of a Chitosanolytic Fungus Penicillium janthinellum LPB-01 on the Hydrolysis of Chitosan
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Koo Bon-Geun
Kim Woo-Jung
Shin Seo-Yoon
Kim Min-Jung
Baek Gu
Kim Tae-Joon
Park Jae-Kweon
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Abstract
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In this study, we investigated the abililty for the degradation of chitosan by fungus isolated from air environment. The fungus that is tentatively named as Penicillium janthinellum LPB-01 based on 26S rDNA sequence analysis and homology search has ability to degrade chitosan to produce chitooligosaccharides. Chitosanolytic activity determined by reducing sugar assay, TLC, and HPLC analysis was confirmed that P. janthinellum LPB-01 actively depolymerize chitosan in both solid-phase and liquid-phase. However, no protein was observed in SDS-PAGE. These results exhibited that P. janthinellum LPB-01 may have constructive chitosanolytic enzyme facing outward of the cell walls. Unexpectedly, however, end-product of the reaction of chitosan with P. janthinellum LPB-01 was defined by HPLC to glucose. In the present study, we can not provide much clear answer why, but it is so worthy to note that there is a so complicated mechanism to catabolite chitosan including chitosanolytic enzyme and deaminase which might be specific for transfer glucosamine to glucose. Nevertheless, these results show that the fungus is suitable for degrading chitooligosaccharides and producing glucose as by product, suggesting the potential use of fungus as biological tool for the catabolic cascade of chitosan.
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KEYWORD
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Penicillium janthinellum, Chitosanolytic fungus, Chitosan, Chitooligosaccharides, Catabolic cascade
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